Coding

Part:BBa_K906003:Design

Designed by: Lucas B. Harrington   Group: iGEM12_WashU   (2012-07-30)


Histidine Patch Thioredoxins (HP-Thioredoxin)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When used as a fusion protein, this protein is often combined with a inducible promoter such as pBAD/araC.


Source

This modified thioredoxin was produced by Zhijian Lu and collegues as described in the paper titled "Histidine Patch Thioredoxins MUTANT FORMS OF THIOREDOXIN WITH METAL CHELATING AFFINITY THAT PROVIDE FOR CONVENIENT PURIFICATIONS OF THIOREDOXIN FUSION PROTEINS" published in 1996 in the Journal of Biological Chemistry. A link to this paper can be found here : [http://www.jbc.org/content/271/9/5059.full]

References